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Original Article

Korean Journal of Medical Physics 2010; 21(2): 209-217

Published online June 25, 2010

Copyright © Korean Society of Medical Physics.

Comparison of Retinal Ganglion Cell Responses to Different Voltage Stimulation Parameters in Normal and rd1 Mouse Retina

정상망막과 변성망막에서 전압자극 파라미터 변화에 따른 망막신경절세포의 반응 비교

Jang Hee Ye*, Sang Baek Ryu†‡, Kyung Hwan Kim†‡, Yong Sook Goo*

예장희*ㆍ류상백†‡ㆍ김경환†‡ㆍ구용숙*

*Department of Physiology, Chungbuk National University School of Medicine, Cheongju, Department of Biomedical Engineering, College of Health Science, Yonsei University, Wonju, Nano Artificial Vision Research Center, Seoul National University Hospital, Seoul, Korea

*충북대학교 의과대학 생리학교실, 연세대학교 보건과학대학 의공학과 신경공학연구실,서울대학교병원 나노인공시각연구센터

Abstract

Retinal prostheses are being developed to restore vision for the blind with retinal diseases such as retinitis pigmentosa (RP) or age-related macular degeneration (AMD). Since retinal prostheses depend upon electrical stimulation to control neural activity, optimal stimulation parameters for successful encoding of visual information are one of the most important requirements to enable visual perception. Therefore, in this paper, we focused on retinal ganglion cell (RGC) responses to different voltage stimulation parameters and compared threshold charge densities in normal and rd1 mice. For this purpose, we used in vitro preparation for the retina of normal and rd1 mice on micro-electrode arrays. When the neural network of rd1 mouse retinas is stimulated with voltage-controlled pulses, RGCs in degenerated retina also respond to voltage amplitude or voltage duration modulation as well in wild-type RGCs. But the temporal pattern of RGCs response is very different; in wild-type RGCs, single peak within 100 ms appears while in RGCs in degenerated retina multiple peaks (∼4 peaks) with ∼10 Hz rhythm within 400 ms appear. The thresholds for electrical activation of RGCs are overall more elevated in rd1 mouse retinas compared to wild-type mouse retinas: The thresholds for activation of RGCs in rd1 mouse retinas were on average two times higher (70.50∼99.87ՌC/cm2 vs. 37.23∼61.65ՌC/cm2) in the experiment of voltage amplitude modulation and five times higher (120.5∼170.6ՌC/cm2 vs. 22.69∼37.57ՌC/cm2) in the experiment of voltage duration modulation than those in wild-type mouse retinas. This is compatible with the findings from human studies that the currents required for evoking visual percepts in RP patients is much higher than those needed in healthy individuals. These results will be used as a guideline for optimal stimulation parameters for upcoming Korean-type retinal prosthesis.

KeywordsRetinal prosthesis, Optimal stimulation parameter, Retinal ganglion cell, rd1 mice, Threshold charge density

Korean Society of Medical Physics

Vol.35 No.2
June 2024

pISSN 2508-4445
eISSN 2508-4453
Formerly ISSN 1226-5829

Frequency: Quarterly

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