Neurochemical Profile Quantification of Regional Adult Mice Brain Using: ex vivo 1H High-Resolution Magic Angle Spinning NMR Spectroscopy

Do-Wan Lee*; Dong-Cheol Woo*; Sung-Ho Lee; Sang-Young Kim*; Goo-Young Kim; Hyang-Shuk Rhim; Chi-Bong Choi; Hwi-Yool Kim; Chang-Wook Lee; Bo-Young Choe*

Article

Original Article

Korean Journal of Medical Physics 2010; 21(1): 35-41

Published online March 25, 2010

Neurochemical Profile Quantification of Regional Adult Mice Brain Using: ex vivo ¹H High-Resolution Magic Angle Spinning NMR Spectroscopy

생체 외 조직 고 분해능 Magic Angle Spinning을 이용한 정상 Adult Mice에서의 뇌 부위별 뇌 신경화학 대사물질 정량분석

Do-Wan Lee*, Dong-Cheol Woo*, Sung-Ho Lee^†, Sang-Young Kim*, Goo-Young Kim^‡, Hyang-Shuk Rhim^‡, Chi-Bong Choi^§, Hwi-Yool Kim^†, Chang-Wook Lee^∥, Bo-Young Choe*

이도완*ㆍ우동철*ㆍ이성호^†ㆍ김상영*ㆍ김구영^‡ㆍ임향숙^‡ㆍ최치봉^§ㆍ김휘율^†ㆍ이창욱^∥ㆍ최보영*

Author Info ⁺

*Department of Biomedical Engineering, Research Institute of Biomedical Engineering, College of Medicine, The Catholic University of Korea, ^†Department of Veterinary Surgery, Konkuk University, ^‡Department of Molecular Biology, College of Medicine, The Catholic University of Korea, ^§Department of Radiology, Kyung-Hee University, ^∥Department of Psychiatry, College of Medicine, The Catholic University of Korea, Seoul, Korea

*가톨릭대학교 의과대학 의공학교실, ^†건국대학교 수의과대학, ^‡가톨릭대학교 의과대학 분자유전학교실, ^§경희의료원 진단방사선과, ^∥가톨릭대학교 의과대학 정신과학교실

Abstract

Go to

Abstract

The purpose of this study is to quantitate regional neurochemical profile of regional normal adult mice brain and assess regional metabolic differences by using ex vivo ¹H high-resolution magic angle spinning nuclear magnetic resonance spectroscopy (¹H HR-MAS NMRS). The animals were matched in sex and age. The collected brain tissue included frontal cortex, temporal cortex, thalamus, and hippocampus. Quantitative 1D spectra were acquired on 40 samples with the CPMG pulse sequence (8 kHz spectral window, TR/TE = 5500/2.2 ms, NEX = 128, scan time: 17 min 20 sec). The mass of brain tissue and D₂O＋TSP solvent were 8~14 mg and 7~13 mg. A total of 16 metabolites were quantified as follow: Acet, NAA, NAAG, tCr, Cr, tCho, Cho, GPC + PC, mIns, Lac, GABA, Glu, Gln, Tau and Ala. As a results, Acet, Cho, NAA, NAAG and mIns were showed significantly different aspects on frontal cortex, hippocampus, temporal cortex and thalamus respectively. The present study demonstrated that absolute metabolite concentrations were significantly different among four brain regions of adult mice. Our finding might be helpful to investigate brain metabolism of neuro-disease in animal model.

Keywords¹H HR-MAS, NMRS, Metabolites, Normal mice, Neurochemical profile