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Original Article

Korean Journal of Medical Physics 2008; 19(3): 157-163

Published online September 25, 2008

Copyright © Korean Society of Medical Physics.

Comparison of Retinal Waveform between Normal and rd/rd Mouse

Jang Hee Ye*, Je Hoon Seo, Yong Sook Goo*

정상 마우스와 rd/rd 마우스의 망막파형 비교

Departments of *Physiology and Anatomy, Chungbuk National University School of Medicine, Cheongju, Korea

충북대학교 의과대학 *생리학교실, 해부학교실

Abstract

Retinal prosthesis is regarded as the most feasible method for the blind caused by retinal diseases such as retinitis pigmentosa or age-related macular degeneration. One of the prerequisites for the success of retinal prosthesis is the optimization of the electrical stimuli applied through the prosthesis. Since electrical characteristics of degenerate retina are expected to differ from those of normal retina, we investigated differences of the retinal waveforms in normal and degenerate retina to provide a guideline for the optimization of electrical stimulation for the upcoming prosthesis. After isolation of retina, retinal patch was attached with the ganglion cell side facing the surface of microelectrode arrays (MEA). 8×8 grid layout MEA (electrode diameter: 30Ռm, electrode spacing: 200Ռm, and impedance: 50 kԹ at 1 kHz) was used to record in-vitro retinal ganglion cell activity. In normal mice (C57BL/6J strain) of postnatal day 28, only short duration (<2 ms) retinal spikes were recorded. In rd/rd mice (C3H/HeJ strain), besides normal spikes, waveform with longer duration (∼100 ms), the slow wave component was recorded. We attempted to understand the mechanism of this slow wave component in degenerate retina using various synaptic blockers. We suggest that stronger glutamatergic input from bipolar cell to the ganglion cell in rd/rd mouse than normal mouse contributes the most to this slow wave component. Out of many degenerative changes, we favor elimination of the inhibitory horizontal input to bipolar cells as a main contributor for a relatively stronger input from bipolar cell to ganglion cell in rd/rd mouse.

KeywordsDegenerate retina, Retinal waveform, Multielectrode array, Bipolar cell, Ganglion cell

Korean Society of Medical Physics

Vol.35 No.4
December 2024

pISSN 2508-4445
eISSN 2508-4453
Formerly ISSN 1226-5829

Frequency: Quarterly

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